Fig. 2

Performance of MethylStar as compared with other BS-Seq analysis pipelines viz. Methylpy, nf-core/methylseq and gemBS in (a) A. thaliana (b) Maize (c) H1 cell line and (d) scBS-Seq samples. CPU processing time taken by METHimpute was not included in the current benchmarking process as there is no equivalent method in the other pipelines to compare with. Because of the very long run times observed for the A. thaliana data, Methylpy and Methylseq were no longer considered for benchmarking of speed in Maize and H1 cell line samples. All pipelines were run using 32 jobs. (e) Peak memory usage as a function of time for 10 random A. thaliana samples. (f) Time taken by each component of MethylStar. X-axis shows the individual components of MethylStar where the dot with lighter shade of orange indicates -without parallel and darker shade of orange indicates - with parallel implementation of MethylStar. On the y-axis is the time in mins. The size of the dot indicates the peak memory usage in MB by each component