Molecular characterization of Pseudomonas from Agaricus bisporus caps reveal novel blotch pathogens in Western Europe

Table 3 In-silico DNA:DNA hybridization of unidentified isolates

Query isolate	Phylo-type (ANI)	Reference strain	dDDH value (%)	Confidence intervals (%)	Difference in GC (%) content
Non-Pseudomonas
B9002	13	Pantoea rodasii LMG 26273^T	40.5	37.5–43.5	0.9
C2002	15	Serratia liquefaciens LMG 7884^T	96.5	95.0–97.6	0.14
C7002	17	Serratia proteamaculans NCPPB 245^T	93.4	91.1–95.1	0.01
P7753	30	Brevundimonas bullata LMG 17157^T	72	68.5–75.2	0.22
P7760	31	Cedecea neteri LMG 7864^T	63	59.7–66.2	0.55
Pseudomonas species
A5002	4	P. yamanorum LMG 27247^T	69.2	65.8–72.5	0.63
IPO3780	4	P. yamanorum LMG 27247^T	70.3	66.8–73.5	0.73
IPO3781	4	P. yamanorum LMG 27247^T	68.2	64.8–71.4	0.59
IPO3782	4	P. yamanorum LMG 27247^T	70.9	67.4–74.1	0.76
J3002	4	P. yamanorum LMG 27247^T	69.1	65.7–72.4	0.67
B6002	9	Isolate P7548 (phylotype 11)	80.9	77.5–83.9	0.24
P7548	11	P. fluorescens DSM 50090^T	57.3	54.1–60.5	0.61
B7002	12	P. edaphica LMG 30152^T	88.2	85.2–90.6	0.03
IPO3747	12	P. edaphica LMG 30152^T	89.9	87.1–92.1	0.1
K6002	12	P. edaphica LMG 30152^T	89.8	87.0–92.0	0.12
K7002	12	P. edaphica LMG 30152^T	89.9	87.1–92.1	0.1
B3002	16	P. reactans LMG 5329^T	44.4	41.4–47.4	2.94
D2002	21	P. fluorescens DSM 50090^T	69.4	65.4–73.0	0.15
IPO3765	25	P. salomonii LMG 22120^T	87.9	84.4–90.7	0.31
IPO3778	26	P. yamanorum LMG 27247^T	64.8	61.5–68.1	0.59
IPO3779	26	P. yamanorum LMG 27247^T	64.8	61.5–68.0	0.6

Only the highest dDDH values from comparing each query strain with multiple reference strains have been reported. Values in bold indicate that the query belongs to the same species as the reference strain, as the confidence intervals meet the 70% threshold for DDH and 1% difference in %GC content. Values that are not highlighted, lie on the border or below the thresholds, and indicate the reference that the query isolate is most closely related to

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