Fig. 4

The relationships between mutated features regulated by CRP, their associations to glycerol as a carbon source, and the mutation effects on their coding sequences. a Mutated features that concurrently converge onto the CRP, Mlc, and GlpR regulons; an asterisk (*) denotes those features found to be mutated with a statistically significant amount relative to all mutations within the ALE experiment (P < 0.05, permutation test). The glpK, crr, and cyaA genes of interest all converge onto the CRP regulon. b A heatmap of the log odds ratios for features from Fig. 4a involved in significant mutation convergence (P < 0.05, permutation test, mutations to feature > 1) and their significantly associated conditions (P < 0.01, Fisher’s exact test). “att” is used to abbreviate “attenuator terminator”. c Heatmap of the Pearson correlation of mutation co-occurrence between cyaA and crr. d The frequency of characterizations to glpK mutations. e Mutation needle plot demonstrating the types of mutations in the amino acid sequence of the gene cyaA. The large light-blue domain is the regulatory domain, the small orange domain is the EIIAGlc binding site, and the yellow domain is the G3P associated inhibition region