Fig. 10

Overexpression of ScADH3 enhances the cold tolerance of transgenic tobacco. a Phenotypes of 2-month-old plants of transgenic line (OE) and the wild type (WT) before and after freezing treatment (− 2 °C for 3 h). b Histochemical staining with DAB (upper panels) for detection of in situ accumulation of H₂O₂ in the transgenic lines (OE) and the wild type (WT) before and after cold treatment. c-e are qRT-PCR expression analysis of NtPOD (c), NtSOD (d), and NtCAT (e) in the (OE) and the wild type (WT). Ubiquitin was used as the reference gene. All data points shown are mean ± SE (n = 3). Different lowercase letters indicate a significant difference, as determined by Duncan’s new multiple range test (p < 0.05)