Fig. 10

Histone epigenetic modification of the WRKY40 gene in the two populations. a. Alignment of the nucleotide sequences of the coding region of the WRKY40 gene in the HK and JN populations. Asterisks indicate the same nucleotide in these two populations. b. ChIP assay of the relative levels of H3K9ac and H3K4me3 in WRKY40 chromatin of the HK and JN plants before and after cold treatment. The primers used in the PCR are shown as bars below the WRKY40 gene. Data are the means±SD of triplicate experiments. Bars with different letters indicate significant differences at P < 0.05 (ANOVA followed by Tukey’s post-hoc test)