Fig. 4
Sweeper and catch tentacles toxic assays and toxins gene expression results. a Differences in the expression of putative toxin-encoding genes between the CT and the ST. The differences in expression of all of the genes with more than 2-fold difference were statistically significant (adjusted p-value < 0.05). b Hemolytic activity is lower in the sweeper tentacles compared to the catch tentacles. Hemolytic units (HU) are shown, normalized to milligram protein. N = 5, t(4) = 3.911, p < 0.001. c Phospholipase A2 (PLA2) activity is higher in sweeper tentacles compared to catch tentacles. PLA2 activity is presented as the number of PLA2 units normalized to milligram protein. N = 5, t(4) = − 3.213, p = 0.032. Boxes are the interquartile range, lines are medians N = 5. d Paralytic activity was detected in catch tentacles but not in sweeper tentacles. The results shown are the PD50 (dose, in micrograms, required to paralyze a ~ 110 mg blowfly larva within 1 min). N = 5