Fig. 5

SOX10 regulates the expression of a novel transcription start site at Arpc1a. a The genomic region surrounding exons 7 and 8 of the rat Arpc1a locus. Y-axes for H3K4me3 and SOX10 ChIP-Seq data: fold enrichment of sequencing reads above chromatin input. Y-axes for Tn5Prime data from rat sciatic nerve, CPT-cAMP- (cAMP) and vehicle-treated (Control) primary Schwann cells, and unmodified and ΔSOX10 S16 cells: number of transcript 5’ends mapped per base, in reads per million. b The 904-base pair ARPC1A Prom 2 is shown along with the position of the two SOX10 dimeric consensus sequences (red bars and red text). The seven species utilized for comparative sequence analysis are shown on the left. cARPC1A Prom 2 (with or without the dimeric SOX10 sequences, as indicated) was tested in luciferase reporter assays in cultured Schwann (S16) cells. Y-axis: fold induction of luciferase activity; error bars indicate standard deviations. Asterisk indicates p < 0.05. d Annotated ARPC1A protein isoforms 1 and 2 contain WD40 repeats (WD, blue) and isoform-specific N-terminal sequences (purple). A cartoon of the predicted protein product of the SOX10-regulated Arpc1a transcript (isoform 3) is presented at the bottom of the panel. Predicted molecular weights based on amino acid sequences are shown in kilodaltons (kDa) on the right