Fig. 6

SOX10-dependent expression of Chn2 transcripts originating at exon 1D. a The genomic region surrounding exon 1B at the rat Chn2 locus. Y-axes for H3K4me3 and SOX10 ChIP-Seq data: fold enrichment of sequencing reads above chromatin input. Y-axes for Tn5Prime data from rat sciatic nerve, CPT-cAMP- (cAMP) and vehicle-treated (Control) primary Schwann cells, and unmodified and ΔSOX10 S16 cells: number of transcript 5’ends mapped per base, in reads per million. b The 844-base pair CHN2 Prom 4 is shown along with the position of the SOX10 dimeric consensus sequence (red bars and red text). The seven species utilized for comparative sequence analysis are shown on the left. cCHN2 Prom 2 (with or without the dimeric SOX10 sequence, as indicated) was tested in luciferase reporter assays in cultured Schwann (S16) cells. Y-axis: fold induction of luciferase activity; error bars indicate standard deviations. Asterisk indicates p < 1 × 10^− 6. d β-chimaerin isoforms 1, 2, and 3. β2-and β3-chimaerins contain Src-homology 2 (SH2, magenta), diacylglycerol binding (C1, blue), and Rac-GTPase activating (Rac-GAP, orange) domains, and are distinguished by isoform-specific N-terminal sequences (red and green). β1-chimaerin includes an isoform-specific N-terminal sequence (purple). Predicted molecular weights based on amino acid sequences are shown in kilodaltons (kDa) on the right