Fig. 8

SOX10-dependent expression of a Gas7 transcription start site at exon 1B. a The genomic region surrounding GAS7 exon 1B at the rat Gas7 locus. Y-axes for H3K4me3 and SOX10 ChIP-Seq data: fold enrichment of sequencing reads above chromatin input. Y-axes for Tn5Prime data from rat sciatic nerve, CPT-cAMP- (cAMP) and vehicle-treated (Control) primary Schwann cells, and unmodified and ΔSOX10 S16 cells: number of transcript 5’ends mapped per base, in reads per million. b The 835-base pair GAS7 Prom 2 is shown along with the position of the SOX10 dimeric consensus sequences (red bars and red text). The seven species utilized for comparative sequence analysis are shown on the left. cGAS7 Prom 2 (with or without the dimeric SOX10 sequences, as indicated) was tested in luciferase reporter assays in cultured Schwann (S16) cells. Y-axis: fold induction of luciferase activity; error bars indicate standard deviations. Asterisk indicates p < 0.005. d GAS7 isoforms a, b, c, and d. Isoform Gas7-c contains Src homology 3 (SH3, green), WW (magenta), and Fes/Cip4 homology (FCH, blue) domains. Gas7-a contains an isoform-specific N-terminal sequence (gold). Predicted molecular weights based on amino acid sequences are shown in kilodaltons (kDa) on the right. e GAS7 protein expression in sciatic nerve, primary Schwann cells, and S16 cells. AARS was used as a protein loading control. Numbered dashes to the left of each blot indicate the position of protein size markers in kilodaltons (kDa). f The intensity of the lower GAS7 band relative to AARS signal in control and cAMP-treated primary Schwann cells. The average across three independent samples is indicated by the bar height. Error bars indicate standard deviation