Comparative genomic analysis of a Shiga toxin-producing Escherichia coli (STEC) O145:H25 associated with a severe pediatric case of hemolytic uremic syndrome in Davidson County, Tennessee, US

Table 1 Genome characteristics of STEC EN1I-0044-2 compared with other highly pathogenic STECs

Chromosome	O145:H25 str. EN1I-0044-2	O145:H25 str. CFSAN004177	O145:H28 str. RM13514	O26:H11 str. 11,368	O103:H2 str. 12,009	O111:H- str. 11,128	O157:H7 str. Sakai
Size (kb)	5131	5191	5586	5697	5449	5371	5498
GC (%)	50.5	50.5	50.7	50.7	50.7	50.6	50.5
CDSs	5149	5193	5613	5364	5054	4972	5230
tRNA	99	96	104	101	98	107	105
Prophages	13	14	20	21	15	17	18
stx genes	stx2a	stx2a	stx2a	stx1	stx1 + 2a	stx1 + 2a	stx1 + 2a
Number of IS^a	55	73	53	62	50	56	37
Integrate elements (IE)	8	9	7	9	6	7	6
LEE-island integration locus	pheU	pheU	selC	pheU	pheV	pheV	selC
pEHEC-like plasmid
Size (kb)	50	52	87	85	76	78	93
GC (%)	47	47	47.6	47.5	49.1	50	47.6
CDSs	79	58	94	98	67	72	85
Number of IS^a	4	4	4	6	6	8	3
Other plasmid(s)
Size (kb)	32/63	35/96	65	63/6/4	N/A	205/98/8/7	3
GC (%)	49/51	49/48	53	52/46/44	N/A	47/48/50/50	43
CDSs	48/86	46/120	69	93/10/3	N/A	222/121/10/10	3
Number of IS^a	4/0	5/0	6	1/0/0	N/A	14/1/0/0	0
Total genome size (bp)	5276	5374	5738	5855	5525	5767	5594

ISSN: 1471-2164