Fig. 2

ASH1L nucleotide conservation. a VISTA plot for Ash1l genomic loci for cow, dog, human, Mus, PO, and rat compared to BW Ash1l. Blue regions indicate exons and red regions indicate conserved non-coding regions. CNS indicates a conserved non-coding sequence in intron three across all mammals analyzed. b DNA sequence alignment for the CNS, indicated in a for the seven mammalian species. Numbers across the top represent nucleotide locations. Conservation graph at the bottom indicates percent nucleotide conservation between the seven mammalian species. Asterisks indicate approximate locations for nucleotide differences between BW and PO. c Regional nucleotide alignment within the CNS. Asterisk indicates a single nucleotide polymorphism between BW and PO. Boxed regions indicate transcription factor binding sites in BW for NKX2.1 and in PO for NBF1