Table 5 Polymorphisms that generate RFLPs between BW and PO in candidate genes that cause spotting in Mus
Candidate Gene | Contig | PCR Amplicon Location | Polymorphism Location | BW sequence | PO Sequence | Forward Primer | Reverse Primer |
|---|---|---|---|---|---|---|---|
Edn3 | NW_006501107 | 1,645,861. .. 1,646,386 | 1,646,160 | AGGCCT (StuI) | AGTCCT | CTCGAGAACCTTGGGATTCA | AACAGGGTCTCCTGCAGTGT |
Ednrb* | NW_006501134 | 1,664,283. .. 1,664,495 | 1,664,389 | CCGG (MspI) | CCAG | ATGACGCCACCCACTAAGAC | GATGATGCCTAGCACGAACA |
Kit | NW_006501162 | 6,362,706. .. 6,363,108 | 6,362,907. .. 6,362,919 | CCGTGGTACCTCTGCTCGGGA (KpnI) | CCGT//GGGA | CCCGTCCTAGCTTTGGAAC | AGCATCAGGGCAACCTTAAA |
Kitl | NW_006501158 | 227,546. .. 228,022 | 227,668 | GGATCC (BamHI) | GAATCC | CCCAATTAGCTGCTCTTCAAAC | GGAGCCTTTGTGTCTTATCAGTA |
Mitf | NW_006501059 | 77,663,262. .. 7,663,281 | 7,663,508 | GTATGC | GCATGC (SphI) | GGATGAGACTCAGGGTGAGG | GCTCCATCACTCGGCATTAT |
Pax3 | NW_006501055 | 3,517,274.. . 3,517,631 | 3,517,515 | TTTAAA (DraI) | TTTCAA | CCTTGCCTACTACGCTCTGA | TAATTCTGCATCCTTCCGGC |
Ret | NW_006501668 | 491,420. .. 491,911 | 491,673 | AGGCCT (StuI) | AGACCT | GTTTCACCCTAGGAAGTTGTGG | GCCTCAGAAGCAGCCCTC |
Snai2 | NW_006502260 | 133,647. .. 133,992 | 133,912 | TTTAAA (DraI) | TTTAAG | CCAAAGTTGAAGGCTGTTGC | AGTCCATTGCTTTCACACCT |
Sox10 | NW_006501150 | 898,573. .. 898,893 | 898,801 | CCAC | CCGC (AciI) | GGCAGACTGAGGGAGGTGTA | GGAGATCAGCCACGAGGTAA |