Table 1 Sequence variants in CDS of BCG-1 (Russia) parent seed lots 361, 367 and 368 and their progeny clinical isolates compared to the reference sequence BCG-1 (Russia) (GenBank: CP013741)
Parent gene BOVR | Parent gene BOVR codon change | Chain | Seed lot 361 | 1986 | 2925 | 4159 | 5075 | 5340 | 5448 | Seed lot 367 | 3363 | Seed lot 368 | 577 | 1032 | Parent gene BOVR amino acid change | Product name | Effect (putative impact) |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
01860 | 509delC | – | CG | CG | CG | CG | CG | CG | CG | CG | C 1.0 | CG | CG | CG | Pro170fs | thioesterase | high |
03020 | 121C > T | – | C | C | C | C | C | T 0.37 | C | C | C | C | C | C | Ala41Thr | PE-PGRS family protein PE_PGRS7 | moderate |
03865 | 685delA | + | CA | CA | CA | CA | CA | CA | CA | CA | CA | C 0.69 | C 0.47 | CA | Thr229fs | methionine aminopeptidase | high |
06950 | 1403 T > C | + | T | T | T | T | T | T | T | T | T | T | C 0.43 | T | Leu468Ser | ATP synthase subunit beta | moderate |
08645 | 2004A > G | + | G 0.42 | A | G 1.0 | A | A | A | A | A | A | A | A | A | Val668Val | polyketide synthase | low |
08855 | 1115A > G | – | G 0.18 | A | A | A | A | A | G 1.0 | A | A | A | A | A | Leu372Pro | amino acid transporter | moderate |
09330 | 982G > A | + | A 0.40 | G | A 0.96 | G | G | G | A 1.0 | G | G | G | G | G | Ala328Thr | type VII secretion integral membrane protein EccD | moderate |
09345 | 128 T > C | + | C 0.33 | T | C 1.0 | T | T | T | C 1.0 | T | T | T | T | T | Ile43Thr | type VII secretion AAA-ATPase EccA | moderate |
10,325 | 634delC | + | GC | GC | GC | GC | GC | GC | GC | GC | GC | GC | GC | G 0.28 | Leu212fs | universal stress protein UspA | high |
10,615 | 587C > T | – | T 0.18 | C | T 1.0 | C | C | C | T 1.0 | C | C | C | C | C | Gly196Asp | dolichol-phosphate mannosyltransferase | moderate |
12,050 | 78C > A | – | A 0.29 | C | C | C | C | C | C | C | C | C | C | C | Leu26Leu | ornithine aminotransferase | low |
13,820- | 755C > T | + | T 0.54 | C | T 1.0 | C | C | C | T 0.89 | C | C | C | C | C | Ala252Val | protease | moderate |
15,090 | 2139A > G | – | G 1.0 | G 1.0 | G 1.0 | G 1.0 | G 1.0 | G 1.0 | G 1.0 | G 1.0 | G 1.0 | G 0.25 | G 0.46 | G 1.0 | Ala713Ala | protein-PII uridylyltransferase | low |
16,925 | 846C > T | – | C | C | C | C | C | C | C | C | C | C | C | T 0.18 | Leu282Leu | N-acetylglucosaminyl-diphospho-decaprenol L-rhamnosyltransferase | low |
17,690 | 833A > G | + | A | A | A | A | A | A | A | A | G 1.0 | A | A | A | Gln278Arg | amidase | moderate |