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Fig. 7 | BMC Genomics

Fig. 7

From: A new mechanism for a familiar mutation – bovine DGAT1 K232A modulates gene expression through multi-junction exon splice enhancement

Fig. 7

Schematic of the two RT-qPCR assays for each junction in DGAT1. The blue boxes represent exons while the blue line represents the intron. The green line represents the probe, while the orange and purple arrows represent the primers for unspliced and spliced mRNA transcripts, respectively. The first assay quantifies the intron containing pre-mRNA transcripts (orange) while the second assay quantifies the spliced mRNA transcripts (purple). The ratio of mRNA:pre-mRNA transcripts is used to generate a splicing efficiency phenotype for each junction

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