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Table 2 Measurements of splicing efficiency at DGAT1 introns 3, 5, 7 and 13 in mammary cell culture for the 232 K and 232A DGAT1 plasmids

From: A new mechanism for a familiar mutation – bovine DGAT1 K232A modulates gene expression through multi-junction exon splice enhancement

Measurement DGAT1 Junction K allele A allele P-value
Splicing ratio 3 0.253 (±0.009) 0.173 (±0.005) 9.37 × 10−4
5 143.18 (±10.23) 133.52 (±9.68) 0.256
7 28.49 (±2.77) 5.55 (±0.74) 9.05 × 10−11
13 92.90 (±5.86) 90.92 (±5.83) 0.411
Spliced mRNA expression 3 0.111 (±0.002) 0.082 (±0.002) 0.015
5 0.946 (±0.3238) 0.421 (±0.160) 0.097
7 0.545 (±0.117) 0.255 (±0.047) 0.009
13 0.984 (±0.219) 0.549 (±0.233) 0.022
Unspliced mRNA expression 3 0.092 (±0.012) 0.0842 (±0.227) 0.376
5 0.286 (±0.0415) 0.180 (±0.0350) 0.069
7 0.216 (±0.030) 0.234 (±0.059) 0.383
13 0.821 (±0.180) 0.503 (±0.124) 0.035
  1. P-values smaller than the Bonferroni threshold (P = 0.0042) are highlighted using bold type