A new mechanism for a familiar mutation – bovine DGAT1 K232A modulates gene expression through multi-junction exon splice enhancement

Table 2 Measurements of splicing efficiency at DGAT1 introns 3, 5, 7 and 13 in mammary cell culture for the 232 K and 232A DGAT1 plasmids

Measurement	*DGAT1* Junction	K allele	A allele	P-value
Splicing ratio	3	0.253 (±0.009)	0.173 (±0.005)	9.37 × 10⁻⁴
	5	143.18 (±10.23)	133.52 (±9.68)	0.256
	7	28.49 (±2.77)	5.55 (±0.74)	9.05 × 10⁻¹¹
	13	92.90 (±5.86)	90.92 (±5.83)	0.411
Spliced mRNA expression	3	0.111 (±0.002)	0.082 (±0.002)	0.015
	5	0.946 (±0.3238)	0.421 (±0.160)	0.097
	7	0.545 (±0.117)	0.255 (±0.047)	0.009
	13	0.984 (±0.219)	0.549 (±0.233)	0.022
Unspliced mRNA expression	3	0.092 (±0.012)	0.0842 (±0.227)	0.376
	5	0.286 (±0.0415)	0.180 (±0.0350)	0.069
	7	0.216 (±0.030)	0.234 (±0.059)	0.383
	13	0.821 (±0.180)	0.503 (±0.124)	0.035

P-values smaller than the Bonferroni threshold (P = 0.0042) are highlighted using bold type

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