Fig. 4

CDR3 nucleotide and predicted amino acid sequences retrieved from the TRB cDNA clones. CDR3-IMGT sequences are shown from codon 105 (the codon after the 2nd-CYS 104 of the V-REGION) to codon 117 (the codon before J-PHE 118 of the J-REGION) according to the unique numbering [33]. The CDR3 nucleotide/amino acid sequence, and the classification of the TRBV and TRBJ genes of each clone are also listed. The TRBV genes retained in TCRVB1 and TCRVB53 clones were assigned to the corresponding germline subgroup only (see text). Nucleotides of the 3’V-REGION and of the 5’J-REGION are indicated in uppercase letters. The sequences considered to present recognizable TRBD genes are indicated in red lowercase letters and the nucleotide substitutions are underlined. The amino acids belonging to a TRBD gene are in bold. Nucleotides that cannot be attributed to any V, D or J region (N-nucleotides), are indicated in lower cases on the left and on the right sides of the TRBD regions. The TRBC gene and the name of each clone are also reported