Fig. 2
From: Leishmania infection induces a limited differential gene expression in the sand fly midgut
Midgut sequencing overall analysis. a. Principal component analysis (PCA) describing the position of each midgut time point on the expression space. Expression space was generated based on the log2 of TPMs using the 10,000 most highly expressed transcripts across libraries. The Eigenvalues and % variance for PC1 and PC3 were 6221.99 and 77.19% and 330.34 and 4.1%, respectively. b-h. Gene expression validation by nCounter (Nanostring). Linear regression analyses comparing the expression profiles of randomly chosen transcripts obtained with RNA-Seq and nCounter (Nanostring) techniques for the seven time points. All comparisons were statistically significant (p < 0.0001). R2: regression coefficient. n: number of transcripts. The color codes labeling each time point were as follow: Aqua (1d); Royal Blue (2d); Sea Green (4d); Sandy Brown (6d); Saddle Brown (8d); Red (12d); and Fuchsia (14d). The triangle and circle shapes represent Leishmania-infected and uninfected samples, respectively