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Fig. 1 | BMC Genomics

Fig. 1

From: RNA-seq analysis of galaninergic neurons from ventrolateral preoptic nucleus identifies expression changes between sleep and wake

Fig. 1

Confirmation for the enrichment of galanin-expressing neurons in eGFP(+) samples collected using LCM. a eGFP(+) samples showed a median 116.9-fold increase in the expression of Gal when compared to the VLPO bulk tissues, indicating effective enrichment of galanin-expressing neurons in our eGFP(+) LCM samples. b Expression of the astrocyte gene Aldh1l1 in both the eGFP(+) and eGFP(−) samples was significantly lower when compared to the VLPO bulk tissues, indicating effective removal of contaminating astrocytes from the neuron samples collected by LCM. Kruskal-Wallis tests were made for comparisons among all three groups. Separate pairwise comparisons were then made between the VLPO bulk tissues and the LCM samples (eGFP+ and eGFP-) using one-tailed Wilcoxon exact test (see Methods). Y-axis shows fold difference in gene expression relative to VLPO bulk tissue on log10 scale

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