Fig. 6

Expression of Fos across multiple time points during SS or SDep in VLPO-Gal neurons and mPFC. a Expression differences of Fos between SS and SDep. In VLPO-Gal, Fos was not significantly different between SS and SDep at earlier time points ZT3 and ZT6, but expressed at a higher level during SDep at ZT9 and ZT12 when compared using a two sample T-test. In mPFC, Fos was significantly elevated by SDep at all four time points. b Pearson’s correlation of Fos expression [log2(CPM + 0.5)] with duration of SS in VLPO-Gal (left) or mPFC (right). In VLPO-Gal, Fos showed a moderately large negative correlation with duration of SS (R = -0.40, p = 0.055), although not statistically significant. In mPFC, Fos was not significantly correlated with duration of SS but the direction of change was opposite from that of VLPO-Gal (R = 0.25, p = 0.23)