Metabolic and transcriptome responses of RNAi-mediated AMPKα knockdown in Tribolium castaneum

BMC Genomics

Table 4 DEGs encoding metabolism related proteins and transcription factors/ co-activators from T. castaneum responding to dsTcAMPKα treatment

Unigene name	padj	Description (blast)	Length (ORF bp)	Log₂Ratio Transcriptome	Log₂Ratio qRT-PCR
Lipid metabolism
FAS1	0.038775298	fatty acid synthase	12,981	1.02	0.37
FAS2	0.056982009	fatty acid synthase	6522	1.76	0.71
FAS3	0.260213539	fatty acid synthase	7152	0.79	1.05
FAS4	0.425672932	fatty acid synthase	6630	0.63	1.16
FAS5	0.170696071	fatty acid synthase	6450	0.58	1.53
ACC	0.186316184	acetyl-CoA carboxylase	7005	0.83	0.10
GPAT3	0.046646995	glycerol-3-phosphate acyltransferases	1440	0.67	1.51
Brummer	1.60E-05	triacylglycerol lipase	1635	−2.20	−0.37
Carbohydrate metabolism
TRE1–1	0.036902117	Trehalase1–1	1662	0.89	1.46
TRE1–3	0.450009964	Trehalase1–3	> 507	0.56	0.58
TRE1–4	0.007152475	Trehalase1–4	1812	0.68	0.59
TRE2	0.036902117	Trehalase2	1647	0.89	1.61
Insulin signaling pathway
IRS1	0.018687502	insulin receptor substrate	2760	1.13	0.63
InR2	0.004419643	insulin-like receptor	4185	1.09	0.23
PI3K	3.37E-08	phosphatidylinositol 4,5-bisphosphate 3-kinase	3186	1.02	0.63
Transcription factor and co-activator
SCAP	0.031742692	sterol regulatory element binding protein cleavage-activating protein	3783	0.81	1.12
SREBP1	0.036908768	sterol regulatory element binding protein 1	3078	0.94	1.74
ChREBP	0.000606393	carbohydrate response-element-binding protein	510	1.18	1.94

ISSN: 1471-2164