Table 2 Significant gene expression changes in the hypothalamo-pituitary-thyroid (HPT) axis between egg production levels. Fold change and significance are presented for key HPT axis genes outside and during the preovulatory surge (RPKM> 0.2, P < 0.05). Negative fold change values represent increased expression in low egg producing hens (LEPH) and positive fold change values represent increased expression in high egg producing hens (HEPH)
Tissue | Gene | Function | Fold | P-Value |
|---|---|---|---|---|
Outside Preovulatory Surge | ||||
Hypothalamus | TRHR | HPT axis signaling | −1.71 | 0.0150 |
TSHB | HPT axis signaling | −10.79 | 0.0368 | |
THRA | thyroid hormone receptor | −2.28 | 0.0274 | |
TTR | thyroid hormone transporter | 3.58 | 0.0166 | |
SLO1C1 | thyroid hormone transporter | −2.45 | 0.0197 | |
Pituitary | CGA | HPT axis signaling | 1.59 | 0.0217 |
DIO2 | thyroid hormone metabolism | −2.14 | 0.0012 | |
SLC5A5 | thyroid hormone synthesis | 6.48 | 0.0109 | |
ATP1B4 | thyroid hormone synthesis | −3.17 | 0.0425 | |
SLC7A5 | thyroid hormone transporter | −1.58 | 0.0088 | |
During Preovulatory Surge | ||||
Hypothalamus | TSHR | HPT axis signaling | −2.07 | 0.0108 |
SLC5A5 | thyroid hormone synthesis | 3.43 | 0.0002 | |
DOUX | thyroid hormone synthesis | 2.44 | 0.0121 | |
SLC26A4 | thyroid hormone synthesis | −1.62 | 0.0186 | |
TTR | thyroid hormone transporter | −76.13 | 1.11E-15 | |
SLC7A5 | thyroid hormone transporter | 1.21 | 0.0359 | |
SLO1C1 | thyroid hormone transporter | −2.83 | 1.34E-07 | |
Pituitary | TSHB | HPT axis signaling | −1.76 | 0.0261 |
DIO2 | thyroid hormone metabolism | −1.61 | 0.0436 | |
TTR | thyroid hormone transporter | −11.21 | 0.0004 | |