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Fig. 1 | BMC Genomics

Fig. 1

From: Comparative transcriptomic analysis of Rickettsia conorii during in vitro infection of human and tick host cells

Fig. 1

Quantitative PCR based validation of R. conorii coding genes differentially expressed during host and tick cell infection in vitro. R. conorii infected HMECs were maintained at either 37 °C or 34 °C, and AAE2 cells infected with R. conorii were maintained at 34 °C for the entire duration of the experiment. Total RNA was extracted at 15 min, 3 h and 24 h post infection, DNaseI treated, and reverse transcribed as described. Expression profile of RC0149 and RC0511 was quantified using gene-specific primers and 16S rRNA as endogenous control. Human and tick cells infected with R. conorii for 15 min served as baseline control and fold changes were calculated as described in methods. Data from three independent replicates is presented as mean ± SEM. ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001

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