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Fig. 4 | BMC Genomics

Fig. 4

From: Comparative transcriptomic analysis of Rickettsia conorii during in vitro infection of human and tick host cells

Fig. 4

Quantitative PCR based validation of R. conorii Rc_sRs highly expressed during tick cell infection in vitro. R. conorii infected HMECs were maintained at either 37 °C or 34 °C, and AAE2 cells infected with R. conorii were maintained at 34 °C for the entire duration of the experiment. Total RNA was extracted at 15 min, 3 h and 24 h post infection, DNaseI treated and reverse transcribed as described. Expression profile of Rc_sR61, sR71 and sR74 which were highly expressed during tick cell infection and identified in our RNA seq data were quantified using sRNA-specific primers and 16S rRNA as housekeeping control. Human and tick cells infected with R. conorii for 15 min served as baseline control and fold changes were calculated as described in methods. Data from three independent replicates is presented as mean ± SEM. ns = not significant, *p < 0.05, **p < 0.01, ***p < 0.001

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