Fig. 3
Verification of sequencing data by RT-qPCR and screening of sequence conserved DELs between yak and cashmere goat. a The expression level of differently expressed lncRNAs and several paired DEGs were detected by RT-qPCR (above), the relative expression levels of lncRNAs and mRNAs were analyzed by the 2−ΔΔCt method and normalized using GAPDH. Data were presented as means ± SEM (n = 3). The same letter means no significant difference, different letters mean significant difference; Comparation of the expression pattern of the sequencing data and RT-qPCR data (below). Log2(fold change) > 0 indicates the transcript up regulated in Jan. (catagen) or Oct. (anagen) compared to Mar.(telogen). Log2(fold change) < 0 is the opposite. b Cluster analysis of the aligned 24 DELs and their paired 23 differently expressed mRNAs in yak. c Heat map showing the expression profiles of DEGs in both yak and cashmere goat during the HFs cycle