Fig. 2

a Genomes clustering based on the presence/absence profile of CMF genes. The two generated clusters are highlighted by gold and skyblue underlying vectors for UMGS and NCBI genomes, respectively. The separation between the two groups is statistically significant (P < 0.001, Fisher’s exact test). b PCA based on Euclidean distances showing a significant separation between NCBI (skyblue) and UMGS (gold) genomes according to the presence/absence profile of CMF genes (P < 0.001, permutation test with pseudo-F ratio). c PCA based on Euclidean distances as presented in panel B) color-coded accordingly to the CheckM score of the analyzed genomes. A gradient of CheckM variation across the two-dimensional space is highlighted. d Biplot showing the significant correlation between CMF (Y- axis) and CheckM scores (X-axis) for the UMGS analyzed (red dots)