Fig. 6

Expression analysis of 12 randomly selected miRNAs by stem–loop qRT-PCR. 18 s rRNA gene was used as the reference gene. Error bars represent the SE for three independent experiments, and three technical replicates were analyzed. Vertical bars indicated LSD values where significant difference were detected (P < 0.05). ‘Ch-3’ with high tiller productive rate and ‘Ch-5’ with few tillers were collected during tillering-stage tall fescue. Pre-tillering and Tillering indicates samples were collected before and after tiller producing stage