Fig. 7

Expression profile of FOXE1 mRNA in the embryonic chicken Müllerian duct. a Time course of FOXE1 mRNA expression in the male and female Müllerian duct during development, as assayed by whole mount in situ hybridization and sectioned whole mounts. Expression is first detected in female and male Müllerian duct at E5.5 (stage 28) and 6.5 (stage 30). At E8.5 (stage 34), FOXE1 is strongly expressed in the developing female ducts, but weak staining is detected in male ducts. Expression is not detected in gonads and mesonephros. Sectioned whole mounts (upper images) show expression of FOXE1 detectable throughout the developmental stages in Müllerian duct mesenchyme (MDM), with no expression in Müllerian duct epithelium (MDE). At E8.5 (stage 34), FOXE1 is strongly expressed in the developing female ducts but weak expression in male ducts. b Quantitative RT-PCR analysis of FOXE1 mRNA expression in E5.5, 6.5 and E8.5 male and female Müllerian ducts. Expression levels were normalized to β-ACTIN and expressed relative to E5.5. Bars represent Mean ± SEM. ** = adjusted p value< 0.01. One-way ANOVA and Tukey’s multiple comparisons test