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Table 1 Criteria for classification of gonadal phases

From: De novo transcriptome assembly from the gonads of a scleractinian coral, Euphyllia ancora: molecular mechanisms underlying scleractinian gametogenesis

Gonad

Phase

The most represented germ cells observed in the gonads

Approximate timings of collection in 2016 to 2017

Ovary

Early

Oocytes with cytoplasmic polarization (<125 μm in diameter)

October, 2016

Middle

Oocytes with accumulation of yolk and other components (126-200 μm in diameter)

December, 2016

Late

Oocytes with accumulation of yolk and other components (201-275 μm in diameter)

February, 2017

Premature/ mature

Oocytes with ‘U’-like germinal vesicles and GVBD (>276 μm in diameter)

April, 2017

Testis

Early

Spermatogonia

February, 2017

Middle

Spermatogonia and primary spermatocytes

March, 2017

Late

Secondary spermatocytes and spermatids

April, 2017

Mature

Sperm

June, 2017