Fig. 5

mRNA expression pattern of PCK1 in muscle and adipose tissue of buffalo and cattle. Comparative analysis of mRNA expression levels in adipose and muscle tissue was performed in buffaloes and cattle by RNA sequencing (a) and RT-qPCR (b). Expression pattern of PCK1 in adipose and muscle of fattened and unfattened animals in buffaloes (c) and in cattle (d). UXT was used to normalize the expression level of PCK1, and their prime sequences were conserved between buffalo and cattle. Expression level data are presented as the mean ± S.E. (RNA sequencing, n = 3; RT-qPCR, n = 6); lower case indicates p < 0.05; upper case indicates p < 0.01