Table 2 Details of primers used in this study
Name | Sense primer (5′–3′) | Antisense primer (5′–3′) | Size (bp) | Use for |
|---|---|---|---|---|
TMEM254 | CGTGCATGCTTGGTATTGGC | GGAACCAGAGCAGTTGGGTC | 116 | qPCR |
MYH3 | GAGGTCACATCCCATCTGCTC | CTACTCATGGTGTTGGCTGAGA | 109 | qPCR |
FABP3 | CGTCTTTCCCAACCTAGCCC | AACCGACACCGAGTGACTTC | 111 | qPCR |
FABP4 | TTTGCTACCAGGAAAGTGGCT | TGACACATTCCAGCACCATCTT | 277 | qPCR |
MYH6 | TGTCGGGGACAGCAGTAAAG | TGTTCAGATTCTCCCGGTGG | 89 | qPCR |
SLC16A6 | TCTTAACATTCACAGCCCCGC | CGATGGCGATGTACATGTGG | 147 | qPCR |
FGF6 | CCCTACAGCCTGCTGGAGATTT | GGCAGGAGGGTCTCTCTGAA | 155 | qPCR |
UCP3 | CGACTCCGTCAAGCAGTTCT | TGGGCAGAATTCCTTTCCAC | 254 | qPCR |
PPARG | GCAAAGTGGAGCCTGTATC | GTAGTGGAACCCTGACGC | 137 | qPCR |
C/EBPα | TGGACAAGAACAGCAACGAG | TTGTCACTGGTCAGCTCCAG | 130 | qPCR |
FABP4 | AAGTCAAGAGCATCGTAA | CCAGCACCATCTTATCAT | 111 | qPCR |
FAT/CD36 | TGGAAAGGACGACATAAGCAAA | TGGAAATGAGGCTGCATCTGT | 118 | qPCR |
GPAT4 | GGGCTTGGGCGTACTCATC | CAGGAACTCCTTGAACCTCCC | 136 | qPCR |
DGAT1 | GGTCGCGGCCTTCGAT | TCTACGTCTCCGTCCTTGTCT | 118 | qPCR |
HSL | CAGTGTCCAAGACAGAGCCA | GCAGCTTCAGGCTTTTGAGG | 102 | qPCR |
LPL | GGCAGCGTGAGTAGGACC | CGGCTTTATCAACTCTATTCT | 147 | qPCR |
PPARα | GCTCCGTTATTACAGACACCC | AACCCTTGCAGCCCTCAC | 180 | qPCR |
UXT | CCGGAAGCCACGGTTCTTA | AGAGATGAAAGCCTCGTAGCG | 133 | qPCR |
PCK1-q | GCGGAGTACAAGGGCAAAGT | CAGGTACTGGCCGAAGTTGT | 81 | qPCR |
PCK1-V | CGGggtaccAGCCAGCAGTTCCATAGCTC | CCGctcgagGCACGGATGCCGAGGTT | 1610 | Vector construction |