Fig. 6

Comparative expression of ACO transcripts. (a) Illustration of differential expression of the two most closely related ACO Glyma models (red: Glyma.09G008400.1 and blue: Glyma.15G112700.1) in the RNA-Seq of the extended hilum regions (EH) or whole seed coat samples (WS) during the indicated developmental stage of either the recurrent parent isoline UC7 (Mi) or the minute hilum isoline UC413 (mi). The average of the RPKMs for two replicates is graphed for the EH10, EH25, and EH50 data. The full data set is shown in Additional file 8a. (b) Graph of RPKMs values of each of the two ACO Glymas from a set of 39 total RNA-Seq libraries representing a range of developmental stages and tissues from the Williams cultivar. See Additional files 8 b,c for the full data of 11 ACO genes and more description of the libraries