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Fig. 6 | BMC Genomics

Fig. 6

From: Identification of diverse cell populations in skeletal muscles and biomarkers for intramuscular fat of chicken by single-cell RNA sequencing

Fig. 6

In situ validation of the mRNA expression of the adipocyte marker APOA1, COL1A1, and ADIPOQ in breast muscle. APOA1 and COL1A1 are selected from the up-regulated genes in intramuscular adipocytes. ADIPOQ is a known adipocyte marker gene and used as a positive control. Fig. a shows the results in the breast muscle of Jingxing-Huang chicken H lines at D5. Fig. b presents the results of those at D66. The co-stained fluorescence images of the three genes are merged. The first row from left to right is a single-channel image of each gene (n = 5). COL1A1 exhibits green fluorescence; APOA1, red fluorescence; and ADIPOQ, blue fluorescence. The blue background is DAPI. The images on the second row from left to right represent the dual-channel merged images of COL1A1 and APOA1, COL1A1 and ADIPOQ, and APOA1 and ADIPOQ

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BMC Genomics

ISSN: 1471-2164

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