Fig. 4

Expression of Glyma.10 g018200 and Glyma.10 g018800 in Kefeng No. 1 and Nannong 1138–2. a qRT-PCR analysis of Glyma.10 g018200 transcription levels in Kefeng No. 1 and Nannong 1138–2. b qRT-PCR analysis of Glyma.10 g018800 transcription levels in Kefeng No. 1 and Nannong 1138–2. The seeds were germinated for 4 days in vermiculite, and uniform seedlings were selected and cultured for 3 days in one-half Hoagland's nutrient solution containing 0.5 mM KH₂PO₄. Finally, they were transferred to +P (0.5 mM KH₂PO₄) and -P (0.005 mM KH₂PO₄) nutrient solution for 15 days and then sampled. Tubulin was used as an internal control. Data were the mean values of biological replicates mean ± standard deviation (SD) (n = 3). Statistical significance was detected by a two-tailed t-test. * and ** significant at 0.05 and 0.01 probability levels, respectively