Fig. 3

Detection of gene transcripts encoding putative secreted proteins by in situ hybridization. Transcripts encoding thirty different genes were localized in the nematode esophageal glands of Pratylenchus penetrans using the corresponding anti-sense DIG-labeled probes. Due to the high variability of the esophageal gland size among different specimens and nematode stages, both dorsal and subventral glands were labelled as esophageal glands. Details regarding each gene annotation and description are presented in the same order as sorted in Table 1. g: esophageal glands; m: median bulb; s: stylet. Bars = 20 μm