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Fig. 6 | BMC Genomics

Fig. 6

From: A new esophageal gland transcriptome reveals signatures of large scale de novo effector birth in the root lesion nematode Pratylenchus penetrans

Fig. 6

Expression profile of Pratylenchus penetrans candidate effector genes in planta. a Semi-quantitative reverse transcription PCR validating the expression levels of 15 gland-cell localized nematode genes on soybean hairy roots at 1, 3, 7 and 12 days after nematode infection. As a positive control, all cDNA libraries were amplified with primers derived from the 18S gene of P. penetrans or the Ubiquitin-3 (UBQ-3) gene of Glycines max. C corresponds to non-infected hairy roots. b Heat maps representing the expression profile of the full set of 53 candidate effectors identified so far for P. penetrans using the fragment per kilobase of transcript per million mapped reads (FPKM) values of the current gland-cell library (Glands), and public messenger RNA-sequencing datasets originating from total RNA extracted from soybean (BioProject ID PRJNA304159 [7]) and alfalfa plants (cultivars Baker and MNGNR-16; BioProject ID PRJNA547347 [13]) infected with the same P. penetrans isolate at 7 DAI. The new candidate effectors identified in this study are represented in bold

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