Fig. 4

PCA score plots (A & B) showing placement of fetal preservation groups distinguished by viral load status (component 1) and viability (component 2). A) Placenta PCA plot. B) Thymus PCA plot. C) Placenta: the top positive and negative loaded genes on component 1 were HIF1A and ISG15, respectively. D) Thymus: the top positive and negative loaded genes on component 1 were ARG2 and C3AR1, respectively. E) Placenta: the top positive and negative loaded genes onto component 2 were PIK3AP1 and IFNW4/W5, respectively. F) Thymus: top positive and negative loaded genes for component 2 in the Thymus were C3AR1 and RHOH, respectively. The log2FC for each group was calculated in contrasted to control (CTRL); uninfected (UNINF), placenta only with viable (PLCO-VIA), PLCO with meconium staining (PLCO-MEC), low viral load with VIA (LVL-VIA), LVL-MEC, high viral load VIA (HVL-VIA) and HVL-MEC. V + F calculated as (LVL-VIA, LVL-MEC, HVL-VIA, and HVL-MEC) contrasted to V - F (UNINF, PLCO-VIA, and PLCO-MEC). MEC + F calculated as (PLCO-MEC, LVL-MEC, and HVL-MEC) contrasted to MEC - F (UNINF, PLCO-VIA, LVL-VIA, and HVL-VIA). Pink is UNINF, purple is PLCO-VIA, dark blue is PLCO-MEC, red is LVL-VIA, yellow is LVL-MEC, blue is HVL-VIA, green is HVL-MEC, orange is V + F, and grey is MEC + F. *indicates significance P < 0.05 calculated in Limma