Differential responses in placenta and fetal thymus at 12 days post infection elucidate mechanisms of viral level and fetal compromise following PRRSV2 infection

Table 2 Number and directionality of DEG by contrast

Contrast (group 1 – group 2)^a		Placenta			Fetal thymus
Group 1	Group 2	Down-Regulated DEG	Up-Regulated DEG	Total DEG	Down-Regulated DEG	Up-Regulated DEG	Total DEG
UNINF	CTRL	0	0	0	0	0	0
PLCO-VIA	CTRL	0	0	0	0	0	0
PLCO-MEC	CTRL	0	0	0	0	0	0
LVL-VIA	CTRL	0	11	11	1	8	9
LVL-MEC	CTRL	25	33	58	2	9	11
HVL-VIA	CTRL	36	46	82	84	30	114
HVL-MEC	CTRL	27	62	89	30	34	64
V + F (LVL-VIA + LVL-MEC + HVL-VIA + HVL-MEC)	V⁻ (UNINF + PLCO-VIA + PLCO-MEC)	77	47	124	97	35	132
MEC + F (PLCO-MEC + LVL-MEC + HVL-MEC)	MEC⁻ (UNINF + PLCO-VIA + LVL-VIA + HVL-VIA)	0	7	7	1	2	3

^aEach group of fetuses was contrasted with the control (mock infected) group. Additionally, virus positive fetuses aka V + F (LVL-VIA + LVL-MEC + HVL-VIA + HVL-MEC) were contrasted with virus negative fetuses aka V - F (UNINF + PLCO-VIA + PLCO-MEC) as well as meconium-stained fetuses aka MEC + F (PLCO-MEC + LVL-MEC + HVL-MEC) versus viable fetuses aka MEC - F (UNINF + PLCO-VIA + LVL-VIA + HVL-VIA)

ISSN: 1471-2164