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Fig. 4 | BMC Genomics

Fig. 4

From: Early response to heat stress in Chinese tongue sole (Cynoglossus semilaevis): performance of different sexes, candidate genes and networks

Fig. 4

Quantitative reverse transcription-polymerase chain reaction (qRT-PCR) validation of RNA-Seq data. Nine DEGs, including hspa1, hsf4, hsc70, eef1akmt4, hsd11b2, hsd17b1, saml, esrrα and ar were selected for qRT-PCR. The left Y-axis represents the relative expression of DEGs, and the right Y-axis represents Fragments Per Kilobase Million (FPKM) determined by RNA-Seq. Data in females, pseudomales and males from each group (control group: CT_F, CT_P and CT_M; heat-stress group: HS_F, HS_P and HS_M) represent as mean ± S.E.M. of three biological replicates (*p < 0.05; **p < 0.01; ***p < 0.001)

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