Fig. 5

PCR and sequence of the fragment between fenE and dacC. a Schematic diagram used to design primers according to conserved bases from NCD-2 and B. velezensis FZB42; b PCR of the fenE-dacC fragment using the genomic templates NCD-2 and FZB42, with 16S rDNA as an internal reference control; c Schematic diagram of the constructed sequencing vector by ligating the fenE-dacC fragment to the pEASY-Blunt Zero vector, d BLAST of the fenE-dacC fragments from NCD-2 and pEASY-Blunt Zero fenE-dacC, in which the two sequences of fenE-dacC were complete same