Genome mining and UHPLC–QTOF–MS/MS to identify the potential antimicrobial compounds and determine the specificity of biosynthetic gene clusters in Bacillus subtilis NCD-2

Table 1 Secondary metabolite gene clusters annotated in B. subtilis NCD-2 using antiSMASH

Cluster	Type	From	To	Most similar known cluster	Similarity	MIBiG BGC-ID^a
cluster 1	NRPS	347,853	413,245	surfactin	82%	BGC0000433_c1
cluster 2	Terpene	1,137,768	1,158,574	–	–	–
cluster 3	NRPS-TransAT PKS-Other KS	1,763,940	1,873,766	bacillaene	100%	BGC0001089_c1
cluster 4	NRPS	1,936,035	2,004,508	fengycin	93%	BGC0001095_c1
cluster 5	Terpene	2,060,609	208,250	–	–	–
cluster 6	T3PKS	2,261,562	2,302,659	–	–	–
cluster 7	NRPS	3,225,454	3,275,189	bacillibactin	100%	BGC0000309_c1
cluster 8	Sactipeptide-head to tail	3,817,363	3,838,974	subtilosin	100%	BGC0000602_c1
cluster 9	Other	3,842,273	3,883,691	bacilysin	100%	BGC0001184_c1

^aIdentification numbers of the most similar gene clusters from B. velezensis FZB42 provided by the MIBiG BGC database. NRPS non-ribosomal peptide synthetase, PKS polyketide synthase, T3PKS type III polyketide, NRPS-TransAT PKS-Other KS, non-ribosomal peptide synthetase-trans-AT polyketide synthase-Other types of polyketide synthase cluster; Sactipeptide-head to tail, head-to-tail cyclised peptide

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