Fig. 6
From: Analysis of splice variants of the human protein disulfide isomerase (P4HB) gene
PCR amplification of the splice junction of splice variants P4HB-02, P4HB-021 and P4HB-027. The specific splice junction of P4HB-02 (89 bp) and 027 (211 bp) were amplified in HCT-116 cells and SK-N-SH cells. For the amplification of P4HB-021, the expected fragment was 148 bp. The amplification of this fragment was performed using a vector pUC57 with the P4HB-021 cloned as template to PCR reaction