Fig. 1
From: DNA methylation in infants with low and high body fatness
The DNA methylation profile of buccal cells fails to separate newborns with high or low body fatness. (a) Outline of experimental design: Buccal cells were isolated from those newborns with the highest (N = 23) and lowest (N = 24) body fatness enrolled the PATHOS (PArents and THeir OffSpring) study. Genomic DNA was isolated from buccal cells, bisulfite-treated and applied to Illumina® Infinium 450 k DNA methylation arrays. (b) Principle components analysis failed to cluster methylation data by fatness (c) Unsupervised hierarchical clustering of the same data also failed to separate subjects by body fatness, sex, fat mass index (FMI) or array. (d) Manhattan plot showing of association of genome-wide DNA methylation levels with infant body fatness. No probes were significantly associated with body fatness after adjusting for multiple correction (FDRADJUSTED = 0.05)