Table 1 Sequencing statistics. H25, H26 and H26 are the three different horse’s DNA analysed. Barcode01 to Barcode09 are the names of the barcodes used in the multiplexing with the kit EXP-NBD-104. The category Unclassified refers to reads that were filtered out at the demultiplexing steps because the barcode sequence was not attributed. exo -: no Exonuclease V treatment; exo A: Exonuclease V 10 U 2 h + REPLI-g 0.2 μM primers; exo B: Exonuclease V 10 U 2 h + REPLI-g 1.0 μM primers

Mean read length (nt)

2209

2248

2004

2733

2526

2553

1821

3241

2869

2145

2379

Median read length (nt)

1706

1763

1579

1918

1815

1865

1440

2336

2172

1545

1741

Read length N50 (nt)

2786

2807

2462

3867

3409

3414

2172

4635

3873

2909

3137

Number of reads

60,619

67,448

60,729

59,028

69,453

55,892

88,175

45,641

55,996

71,073

634,054

% of Total reads

10%

11%

10%

9%

11%

9%

14%

7%

9%

11%

100%

Total bases (nt)

133,906,674

151,599,799

121,723,567

161,303,032

175,444,343

142,699,042

160,526,846

147,900,066

160,661,882

152,461,373

1,508,226,624

% of Total bases

9%

10%

8%

11%

12%

9%

11%

10%

11%

10%

100%

Mean read quality

11.5

11.5

11.5

10.9

10.9

10.9

11.3

11.4

11.5

6.8

10.8

Median read quality

11.7

11.8

11.7

11.1

11.2

11.1

11.6

11.7

11.8

5.8

11.3