Table 1 Validation method parameters for quantification of seven steviol glycosides

LOD (μg/ml)

7.3

3.1

1.6

2.3

2.8

3.4

3.1

LOQ (μg/ml)

20.1

9.7

6.4

9.2

10.8

12.2

10.6

Calibration curve

y = 266.95x + 25.022

y = 219.28x + 1.9539

y = 222x + 2.7051

y = 170.87x-2.5236

y = 166.02x + 6.0046

y = 160.66x + 8.6147

y = 166.18x + 11.745

Mean correlation coefficient (R2)

0.9988

0.9989

0.9992

0.9991

0.9991

0.9995

0.9994

Linear range (μg/ml)

20.1–597.0

19.4–568.1

25.6–3610.4

33.7–586.0

21.7–4820.0

14.4–301.0

15.5–567.0

Accuracy (%, n = 3)

87.3

108.1

96.5

104.3

97.4

85.6

103.8

Injection precision (RSD%, n = 6)

1.97

1.94

1.53

2.03

1.72

1.58

2.34

Stability (RSD%)

1.51

1.73

1.21

1.93

1.05

1.46

2.01

System suitability (RSD%, n = 6)

1.92

1.85

1.74

2.13

1.59

1.95

1.87