Fig. 1
From: jouvence, a new human snoRNA involved in the control of cell proliferation
Structure, expression, and rRNA target of the human snoRNA-jouvence. a Predicted secondary structure of the h-jou, as determined by the RNAfold web server (ViennaRNA Web Services, Vienna University, Austria) (http://rna.tbi.univie.ac.at/). Though h-jou has a primary sequence in agreement with the H/ACA boxes, its secondary structure does not show a typical double hairpin. Nucleotides highlighted in pink/red correspond to the two putative H (ANANNA) boxes, the nucleotides highlighted in pale-blue are involved in the recognition of the rRNA target, while the nucleotides highlighted in pale-green correspond to the ACA box. b Predicted 18S-rRNA target showing the complementary sequences of the two parts of the loop, as well as the putative pseudouridylation site (U in position 1397). c Expression level (dCT) (dCT = Ct snoRNA - Ct GAPDH) in various cell lines determined by RT-qPCR (Taqman). Compared to the standard reference gene GAPDH, h-jou is weakly expressed in all different tested cell lines, with a very weak expression (to the limit of detection) in the U87 line (glioblastoma), and the strongest expression in the HEK293 cells (human embryonic kidney)