Fig. 2

The overexpression of h-jou increases the proliferation of the cells. a Cells number of HCT116 stably transfected cells with jouvence (in plasmid pcDNA-3.1) compared to the empty vector transfected cells determined by VicellXR. b Same batch of cells than A, but spread and grow in 96 cell-plates and evaluated by luminescence (CellTiter-Glo), which quantify the amount of ATP, and consequently but indirectly, the number of cells. c Cells number of HEK293 stably transfected cells with jouvence compared to the empty vector transfected cells determined by VicellXR. d Same batch of cells than D, but spread and growth in 96 cell-plates and evaluated by luminescence (CellTiter-Glo). e Cells number of Caco-2 stably transfected cells with jouvence compared to the empty vector transfected cells determined by VicellXR. The cells overexpressing jouvence are more numerous after 24 h post-seeding, which have doubled their number after 72 h, and again, more than tripled after 160 h. f, g, h Expression level (Fold change) of the transfected snoRNA-jouvence (overexpression) compared to non-transfected cells. f In HCT116, h-jou is increased by about 350-fold. g In Caco-2, the expression level of h-jou is increased by about 200-fold. h In HEK293, the expression level is increased by about 1200-fold. Statistics: For the VicelXR (A-C-D) n = 2, for the Luminescence CellTiter-Glo (B-D), n = 6. Each figure is representative of three independent experiments. (p-values: * p < 0,05; ** p < 0,005; *** p < 0,0005). Errors bars represent the mean +/− S.E.M. (p-value were calculated using the one-tail unpaired t-test using GraphPad Prism)