Fig. 10

Distribution of crosslink sites in annotated genes and their UTRs. a Positions of crosslink sites around the start of annotated genes. Negative nt positions correspond to 5′-UTRs, positive positions are located in the genes. One hundred nt upstream and downstream of the gene starts were analyzed. b Logo of the sequence motif that was detected by meme for the peak at position − 30 in the 5‘-UTR. c Positions of crosslink sites around the end of annotated genes. Negative nt positions are located in the genes, positive positions correspond to the 3’-UTRs. One hundred nt upstream and downstream of the gene ends were analyzed. d Model for circular degradation of RNA by the archaeal exosome. RNA is degraded exoribonucleolytically in 3′-5′-direction in a processive manner, and nucleoside diphosphates (NDPs) are released. During the degradation process, the 5′-end of the RNA substrate is bound to the exosome. In the iCLIP analysis, this results in an even distribution of crosslink sites in the RNA body and clusters of crosslink sites in the 5′-region. Preferential binding of poly(A) stretches is indicated