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Fig. 5 | BMC Genomics

Fig. 5

From: The piperazine compound ASP activates an auxin response in Arabidopsis thaliana

Fig. 5

Effect of ASP on auxin response in Arabidopsis. Seedlings grown on 1/2 MS agar medium with DMSO for 5 days and then transferred into Eppendorf tube (10 seedlings per tube) containing 1 ml of 1/2 MS liquid medium supplied with DMSO, 1 μM indole-3-acetic acid (IAA) and 5 μM ASP and incubated for 24 h. After ten hours of β-glucuronidase (GUS) staining seedlings were cleared for microscopy analysis. GUS- expressing in cotyledon (a), root/ stem transition (b), and root tip (c) treated for 24 h with DMSO (Left), 1 μM IAA (Middle) and 5 μM ASP (Right). Photographs show representative individuals from at least 20 stained plants (Scale bar = 100 μm)

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