Fig. 5

Fatty acid metabolic pathways a Summary of major changes in fatty acid synthesis pathway during O. sinensis morphotype change from proliferative (BP) to stationary (BS) stage. Compounds with black, solid borders represent metabolites identified in the present study, whereas those without borders were not identified herein. The red and blue text indicate significantly up-regulated and down-regulated compounds, respectively relative to BP with BS. Italicized text indicates up-regulated mRNA (red) or down-regulated mRNA (blue). b Expression patterns for genes involved in fatty acid synthesis at different stages of O. sinensis as detected by qRT-PCR. Each bar represents a mean ± SEM of three replicates, while letters on the bars indicate significance level based on P < 0.05. c Expression patterns of fatty acid degradation genes based on FPKM values from transcriptome data. ACO: aconitase; ACLY: ATP-citrate lyase; ACC: acetyl-CoA carboxylase; FASN: fatty acid synthase; ACOT: acyl-CoA thioesterase; FADS9: fatty acid delta 9 desaturase; FADS12: fatty acid delta 12 desaturase; PGD: phosphogluconate dehydrogenase; G6PD: glucose-6-phosphate 1-dehydrogenase; ME: malic enzyme; PK: pyruvate kinase