Fig. 4

PDGFD expression is associated with adipogenesis and higher in adipose tissues of lean than that in fat individuals in different species. a Expression of several marker genes involved in adipogenesis in tail tissues of fat-tailed sheep at different stages of embryonic development revealed by RNA-seq. PDGFD expression in tail tissues of fat-tailed sheep at different stages of embryonic development revealed by b RNA-seq and c qPCR. d Heatmap showing the correlation matrix between expression of PDGFD and the indicated adipogenesis markers. e Expression of PDGFD in different cell fractions of human subcutaneous adipose tissues. Macrophages were subdivided into M1 and M2 (GSE100795). f Expression of Pdgfd during the adipogenesis of mouse 3 T3-L1 preadiopocytes induced by ω-3 fatty acid DHA revealed by RNA-seq (GSE118471). g PDGFD expression is higher in fat tissues of thin-tailed and fat-tailed sheep at E70 and h adult stage revealed by qPCR. *P < 0.05. i Pdgfd expression is higher in inguinal fat of high weight gaining mice than that of in low weight gaining mice revealed by microArray analysis (GSE4692). *P < 0.05 (n = 3). PDGFD expression is higher in adipocytes from lean than that from obese Indian individuals in both j male and k female subjects revealed by microarray (GSE2508)